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Moritz NegwerWhole-mouse imaging the hard way: Clearing, then slicing + block-face imaging with beam-scan / sensor scan-line synchronization and continuous stage movement in an oblique imaging setup (ViSOR). This time, they scaled it up to whole-mouse and used pre-cleared mouse bodies (ARCHmap-blockface-ViSOR): High-speed mapping of whole-mouse peripheral nerves at subcellular resolution Shi et al., preprint at biorxiv 2025 <a href="https://doi.org/10.1101/2025.01.22.632569" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1101/2025.01.22.632569</a> <a href="https://mstdn.science/tags/neuroscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#neuroscience</a> <a href="https://mstdn.science/tags/lightsheet" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#lightsheet</a> <a href="https://mstdn.science/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#microscopy</a> <a href="https://mstdn.science/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a>

Philip HubbardHere's a <a href="https://fediscience.org/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> segmentation of some neurons from the optic lobe, or visual processing area of the <a href="https://fediscience.org/tags/Drosophila" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Drosophila</a> fruit fly's brain. The <a href="https://fediscience.org/tags/connectome" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#connectome</a> for the full optic lobe was released in 2024 by <a href="https://fediscience.org/tags/HHMIJanelia" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#HHMIJanelia</a>, Google, and the University of Cambridge, with support from the Wellcome Trust.

Philip HubbardThis segmentation of <a href="https://fediscience.org/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> data reconstructs neurons of the "MANC", or male adult nerve cord, from the <a href="https://fediscience.org/tags/Drosophila" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Drosophila</a> fruit fly. The nerve cord is like a spinal cord and connects the brain and muscles. <a href="https://fediscience.org/tags/HHMIJanelia" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#HHMIJanelia</a>, Google, and the University of Cambridge released this work in 2023.

Philip HubbardA use of <a href="https://fediscience.org/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> data is making segmentations. Here's one, of <a href="https://fediscience.org/tags/Drosophila" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Drosophila</a> protocerebral bridge and nodulus neurons, from the 2020 Hemibrain by <a href="https://fediscience.org/tags/HHMIJanelia" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#HHMIJanelia</a> and Google. Michał Januszewski and Viren Jain's team made an initial segmentation with <a href="https://fediscience.org/tags/AI" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#AI</a> flood-fill algorithms...

Furqan Shah<a href="https://mstdn.science/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> – No this is NOT an aerial view of the Grand Canyon.This is a scanning electron microscopy image of the cranial sutures in the rat skull. 🔬💀🐀 These are fibrous joints where the bones of the skull interdigitate, allowing for flexibility and growth during infancy and early childhood before fusing in adulthood.🔬🦴🧪<a href="https://mstdn.science/tags/bone" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#bone</a> <a href="https://mstdn.science/tags/electronmicroscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#electronmicroscopy</a> <a href="https://mstdn.science/tags/imaging" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#imaging</a> <a href="https://mstdn.science/tags/biomaterials" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biomaterials</a> <a href="https://mstdn.science/tags/science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#science</a> <a href="https://mstdn.science/tags/medicine" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#medicine</a> <a href="https://mstdn.science/tags/biology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biology</a> <a href="https://mstdn.science/tags/development" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#development</a> <a href="https://mstdn.science/tags/osteology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#osteology</a> <a href="https://mstdn.science/tags/evolution" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#evolution</a>

Katrina VelleHappy <a href="https://mstdn.science/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a>! Here is a kymograph of a cell crawling into a microchannel. Each horizontal row of pixels represents a line drawn from outside (green) through a channel (purple) at 5 second timepoints. ~1/2 way down (20 min) a cell fully enters the channel and takes off!

David J Solecki🌟 Pre-vacation <a href="https://mstdn.party/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> magic rendered by @Daniel_Stabley! 🌟 This is a showstopper: our top plasma membrane probes light up neurons and glia. But wait, there's more! The grand finale? A lattice light sheet stunner showing off its isotropic prowess in the XZ view. Confocal 3D rotations, eat your heart out! 🧠🔬✨ <a href="https://mstdn.party/tags/Neuroscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Neuroscience</a> <a href="https://mstdn.party/tags/MicroscopyMagic" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMagic</a> <a href="https://mstdn.party/tags/NextLevelImaging" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#NextLevelImaging</a> <a href="https://mstdn.party/tags/cellbio" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cellbio</a> <a href="https://mstdn.party/tags/cellfie" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cellfie</a> <a href="https://mstdn.party/tags/scicomm" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#scicomm</a> <a href="https://mstdn.party/tags/neuroscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#neuroscience</a> <a href="https://mstdn.party/tags/Microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Microscopy</a>

MEActNOW⚠️ That's what a leaky blood vessel looks like in the microscope. Astrocytes (🟡Gfap) around the blood vessel become reactive, inducing neuroinflammatory responses and attacking the surrounding neurons (🔵Neun) in the pathway. <a href="https://mastodon.social/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> <a href="https://mastodon.social/tags/LongCovid" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#LongCovid</a>

Katrina VelleHappy <a href="https://mstdn.science/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a>!

André LampeIch habe ein bisschen USB 3.0 Kabel zerschnitten und Querschnittsbilder mit dem Mikroskop gemacht. Das Video hat Kapitelmarken für die Eiligen und noch mehr Infos und Links in der Videobeschreibung. Viel Freude! <a href="https://chaos.social/tags/Mikroskop" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Mikroskop</a> <a href="https://chaos.social/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> <a href="https://chaos.social/tags/dieKleinenDinge" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#dieKleinenDinge</a> <a href="https://youtu.be/iIYT0736AQQ?feature=shared" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://youtu.be/iIYT0736AQQ?feature=shared</a>

André LampeEs ist zwar nicht Montag, aber ich hab lange nichts mehr zu <a href="https://chaos.social/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> gepostet, also: Querschnitt durch ein USB3 Kabel, hier zu sehen einer der zwei Zusätzlichen DatenKanäle im USB3 Standard (lila & Orange) mit unisolierter zusätzlicher Schirmung. <a href="https://chaos.social/tags/Miroskop" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Miroskop</a> <a href="https://chaos.social/tags/Mikroskopie" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Mikroskopie</a> <a href="https://chaos.social/tags/dieKleinenDinge" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#dieKleinenDinge</a>

Christophe Leterrier 🔬Let's have a <a href="https://mas.to/tags/notthecover" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#notthecover</a> for <a href="https://mas.to/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a>: hippocampal neuron with endogenously tagged HA-actin — one of the proposed covers for <a href="https://rupress.org/jcb/article-abstract/222/10/e202208110/276185/Presynapses-contain-distinct-actin" rel="nofollow noopener noreferrer" target="_blank">https://rupress.org/jcb/article-abstract/222/10/e202208110/276185/Presynapses-contain-distinct-actin</a>

André LampeEs ist Montag, also auch <a href="https://chaos.social/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> Im Video seht ihr eine Mikroskopie-Aufnahme von einem Mehrklingen-Rasierer, den ich langsam kippe, so dass man die Schneide-Kante einer der Klingen gut erkennen kann. Das Video stammt aus einem ganzen Mikroskopie-Stream über Messer und Klingen, hier findet ihr einen Zusammenschnitt davon: <a href="https://www.youtube.com/watch?v=9LRrt-hsuYk" rel="nofollow noopener noreferrer" target="_blank">https://www.youtube.com/watch?v=9LRrt-hsuYk</a>

André LampeHeute ist Montag, also auch <a href="https://chaos.social/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> Ich habe mir mal Stifte und die entsprechenden Linien auf Papier unter dem <a href="https://chaos.social/tags/Mikroskop" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Mikroskop</a> angeschaut, und das ganze sogar live gestreamt. Auf den Bildern unten seht ihr die Striche auf Papier, eine Kugelschreiber-"Spitze" und eine Nahaufnahme eines Farbdrucks. Maßstab jeweils im Bild. Bilder CC BY 4.0Zusammenschnitt des Streams: <a href="https://youtu.be/sXN1yjkmCik?feature=shared" rel="nofollow noopener noreferrer" target="_blank">https://youtu.be/sXN1yjkmCik?feature=shared</a>

André LampeHeute ist Montag, also auch <a href="https://chaos.social/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a> Ich habe mir mal die grauen Reflektorstreifen einer Warnweste unter dem Mikroskop angesehen (war in einem früheren Mikroskop-Stream auf <a href="https://chaos.social/tags/twitchDE" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#twitchDE</a>) - diese Reflektorstreifen bestehen aus kleinen Glaskügelchen mit einem Durchmesser von ca. 40-70µm, eingebettet in eine Art Gel.Bilder sind CC BY 4.0

Katrina VelleHappy (or not?) <a href="https://mstdn.science/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a>! I made a chart out of all the amoeba faces I've found to put on the lab fridge. Unfortunately, happy faces are not an observable phenotype for Naegleria.

Katrina VelleBetter late than never for <a href="https://mstdn.science/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a>! Here is a z-stack (every focal plane moving vertically through a sample) of actin polymer-stained Naegleria amoebae, followed by a maximum intensity projection (the brightest xy point of all the z positions). The darker colors here represent places where there is more actin polymer.

Katrina VelleHappy <a href="https://mstdn.science/tags/MicroscopyMonday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#MicroscopyMonday</a>! Here's a movie from the "Titus Takeover" week, where Meg Titus and several million Dictyostelium cells visited the Fritz-Laylin Lab! Here are 2 movies (1st at 4x, 2nd at 10x) of Dicty cells streaming to form mounds during overnight starvation.

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