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Bose-Einstein-KondensatA <a href="https://mstdn.social/tags/RaspberryPi" class="mention hashtag" rel="nofollow noopener" target="_blank">#RaspberryPi</a>-<a href="https://mstdn.social/tags/automated" class="mention hashtag" rel="nofollow noopener" target="_blank">#automated</a> <a href="https://mstdn.social/tags/OpenSource" class="mention hashtag" rel="nofollow noopener" target="_blank">#OpenSource</a> milligram-scale, four channel <a href="https://mstdn.social/tags/protein" class="mention hashtag" rel="nofollow noopener" target="_blank">#protein</a> <a href="https://mstdn.social/tags/purification" class="mention hashtag" rel="nofollow noopener" target="_blank">#purification</a> system based on liquid <a href="https://mstdn.social/tags/chromatography" class="mention hashtag" rel="nofollow noopener" target="_blank">#chromatography</a>:-<a href="https://mstdn.social/tags/GUI" class="mention hashtag" rel="nofollow noopener" target="_blank">#GUI</a> & <a href="https://mstdn.social/tags/Python" class="mention hashtag" rel="nofollow noopener" target="_blank">#Python</a> API<a href="https://doi.org/10.1101/2023.08.09.552685" rel="nofollow noopener" translate="no" target="_blank">https://doi.org/10.1101/2023.08.09.552685</a> <a href="https://mstdn.social/tags/DIYbio" class="mention hashtag" rel="nofollow noopener" target="_blank">#DIYbio</a> <a href="https://mstdn.social/tags/lab" class="mention hashtag" rel="nofollow noopener" target="_blank">#lab</a> <a href="https://mstdn.social/tags/instruments" class="mention hashtag" rel="nofollow noopener" target="_blank">#instruments</a> <a href="https://mstdn.social/tags/LC" class="mention hashtag" rel="nofollow noopener" target="_blank">#LC</a> <a href="https://mstdn.social/tags/FPLC" class="mention hashtag" rel="nofollow noopener" target="_blank">#FPLC</a>

Bose-Einstein-Kondensat<a href="https://mstdn.social/tags/Colosseum" class="mention hashtag" rel="nofollow noopener" target="_blank">#Colosseum</a>: A <$100, <a href="https://mstdn.social/tags/3Dprinted" class="mention hashtag" rel="nofollow noopener" target="_blank">#3Dprinted</a>, scalable & <a href="https://mstdn.social/tags/Arduino" class="mention hashtag" rel="nofollow noopener" target="_blank">#Arduino</a>-<a href="https://mstdn.social/tags/automated" class="mention hashtag" rel="nofollow noopener" target="_blank">#automated</a> <a href="https://mstdn.social/tags/OpenSource" class="mention hashtag" rel="nofollow noopener" target="_blank">#OpenSource</a> fraction collector:Paper: <a href="https://doi.org/10.1016/j.ohx.2021.e00201" rel="nofollow noopener" target="_blank">https://doi.org/10.1016/j.ohx.2021.e00201</a> YouTube: <a href="https://www.youtube.com/watch?v=yG7ECh5GO0o" rel="nofollow noopener" target="_blank">https://www.youtube.com/watch?v=yG7ECh5GO0o</a> Build instructions: <a href="https://doi.org/10.5281/zenodo.4677604" rel="nofollow noopener" target="_blank">https://doi.org/10.5281/zenodo.4677604</a><a href="https://mstdn.social/tags/DIYbio" class="mention hashtag" rel="nofollow noopener" target="_blank">#DIYbio</a> <a href="https://mstdn.social/tags/lab" class="mention hashtag" rel="nofollow noopener" target="_blank">#lab</a> <a href="https://mstdn.social/tags/instruments" class="mention hashtag" rel="nofollow noopener" target="_blank">#instruments</a> <a href="https://mstdn.social/tags/fluidics" class="mention hashtag" rel="nofollow noopener" target="_blank">#fluidics</a> <a href="https://mstdn.social/tags/LC" class="mention hashtag" rel="nofollow noopener" target="_blank">#LC</a> <a href="https://mstdn.social/tags/HPLC" class="mention hashtag" rel="nofollow noopener" target="_blank">#HPLC</a> <a href="https://mstdn.social/tags/FPLC" class="mention hashtag" rel="nofollow noopener" target="_blank">#FPLC</a> <a href="https://mstdn.social/tags/chromatography" class="mention hashtag" rel="nofollow noopener" target="_blank">#chromatography</a>

Sebastian S. Cocioba 🌻🛠Found a really nice <a href="https://genomic.social/tags/opamp" class="mention hashtag" rel="nofollow noopener" target="_blank">#opamp</a> to design my <a href="https://genomic.social/tags/UVC" class="mention hashtag" rel="nofollow noopener" target="_blank">#UVC</a> detector for my <a href="https://genomic.social/tags/bespoke" class="mention hashtag" rel="nofollow noopener" target="_blank">#bespoke</a> <a href="https://genomic.social/tags/FPLC" class="mention hashtag" rel="nofollow noopener" target="_blank">#FPLC</a> project. It has really low input bias current and offset <a href="https://genomic.social/tags/voltage" class="mention hashtag" rel="nofollow noopener" target="_blank">#voltage</a>. It's a bit slow with a slew rate of about 0.35v/us (would take about 14 <a href="https://genomic.social/tags/microseconds" class="mention hashtag" rel="nofollow noopener" target="_blank">#microseconds</a> to rise from 0 to 5v) but, for my needs, this is perfect. Also found a better <a href="https://genomic.social/tags/UVC" class="mention hashtag" rel="nofollow noopener" target="_blank">#UVC</a> detector that has high sensitivity around 280nm. Time to draft a <a href="https://genomic.social/tags/transimpedence" class="mention hashtag" rel="nofollow noopener" target="_blank">#transimpedence</a> amplifier <a href="https://genomic.social/tags/circuit" class="mention hashtag" rel="nofollow noopener" target="_blank">#circuit</a> <a href="https://genomic.social/tags/PCB" class="mention hashtag" rel="nofollow noopener" target="_blank">#PCB</a>!

Sebastian S. Cocioba 🌻🛠I wrote up today's findings in my research blog. Made a simple desalting column out of parts from McMaster which looks promising. All parts are hotlinked in the post so feel free to try your hand as well. Lots of room for improvement. Enjoy!<a href="https://genomic.social/tags/FPLC" class="mention hashtag" rel="nofollow noopener" target="_blank">#FPLC</a> <a href="https://genomic.social/tags/chromatography" class="mention hashtag" rel="nofollow noopener" target="_blank">#chromatography</a> <a href="https://genomic.social/tags/diybio" class="mention hashtag" rel="nofollow noopener" target="_blank">#diybio</a> <a href="https://genomic.social/tags/protein" class="mention hashtag" rel="nofollow noopener" target="_blank">#protein</a> <a href="https://genomic.social/tags/desalting" class="mention hashtag" rel="nofollow noopener" target="_blank">#desalting</a> <a href="https://genomic.social/tags/column" class="mention hashtag" rel="nofollow noopener" target="_blank">#column</a> <a href="https://genomic.social/tags/handmade" class="mention hashtag" rel="nofollow noopener" target="_blank">#handmade</a> <a href="https://genomic.social/tags/bespoke" class="mention hashtag" rel="nofollow noopener" target="_blank">#bespoke</a><a href="https://www.atinygreencell.com" rel="nofollow noopener" target="_blank">https://www.atinygreencell.com</a>

Sebastian S. Cocioba 🌻🛠Fused silica tubing for UV detector of my crappy bespoke FPLC. Gonna wait for silicone to set, add another coat to reinforce the connection, and then 3D print a holder for the UVC sensor and 280nm LED. Gonna make a constant current driver later...<a href="https://genomic.social/tags/fplc" class="mention hashtag" rel="nofollow noopener" target="_blank">#fplc</a> <a href="https://genomic.social/tags/diybio" class="mention hashtag" rel="nofollow noopener" target="_blank">#diybio</a> <a href="https://genomic.social/tags/laboratoryHardware" class="mention hashtag" rel="nofollow noopener" target="_blank">#laboratoryHardware</a> <a href="https://genomic.social/tags/bespoke" class="mention hashtag" rel="nofollow noopener" target="_blank">#bespoke</a> <a href="https://genomic.social/tags/handmade" class="mention hashtag" rel="nofollow noopener" target="_blank">#handmade</a> <a href="https://genomic.social/tags/protein" class="mention hashtag" rel="nofollow noopener" target="_blank">#protein</a> <a href="https://genomic.social/tags/UVC" class="mention hashtag" rel="nofollow noopener" target="_blank">#UVC</a> <a href="https://genomic.social/tags/ultraviolet" class="mention hashtag" rel="nofollow noopener" target="_blank">#ultraviolet</a> <a href="https://genomic.social/tags/3dprinting" class="mention hashtag" rel="nofollow noopener" target="_blank">#3dprinting</a>

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