Fabrizio Musacchio<p><a href="https://sigmoid.social/tags/Janelia" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Janelia</span></a> is offering a 12-day bootcamp designed to demonstrate how biological queries and hypotheses steer experimental designs on various <a href="https://sigmoid.social/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a> platforms and across length scales from molecules to small animals:</p><p>⏰ J uly 28 - August 9, 2024<br>⏰ Deadline to apply: Feb 15 (11:59 p.m. ET)<br>🌍 <a href="https://www.janelia.org/you-janelia/conferences/bootcamp-course-optical-imaging-across-biological-length-scales?Email_Preferences_Subscription_Center=&mc_cid=a9a3274574&mc_eid=fb2da94283" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">janelia.org/you-janelia/confer</span><span class="invisible">ences/bootcamp-course-optical-imaging-across-biological-length-scales?Email_Preferences_Subscription_Center=&mc_cid=a9a3274574&mc_eid=fb2da94283</span></a></p><p><a href="https://sigmoid.social/tags/SuperResolutionMicroscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>SuperResolutionMicroscopy</span></a> <a href="https://sigmoid.social/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> <a href="https://sigmoid.social/tags/LightSheetMicroscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>LightSheetMicroscopy</span></a> <a href="https://sigmoid.social/tags/ImageProcessing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>ImageProcessing</span></a> <a href="https://sigmoid.social/tags/DataAnalysis" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>DataAnalysis</span></a> <a href="https://sigmoid.social/tags/FOSS" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>FOSS</span></a> <a href="https://sigmoid.social/tags/BigData" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>BigData</span></a></p>
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#STED
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Patrick van der Wel<p>Now open for registrations: short course on single molecule techniques in molecular <a href="https://mastodon.social/tags/biophysics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>biophysics</span></a>, as part of the <a href="https://mastodon.social/tags/MOSBRI" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>MOSBRI</span></a> network. This course is hosted at the University of <a href="https://mastodon.social/tags/Groningen" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Groningen</span></a> by our colleagues of the molecular biophysics group. NOTE deadline: Sept 15th for registration! Course itself is in early November: <a href="https://www.mosbri.eu/training/end-user-short-courses/esc7/" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">mosbri.eu/training/end-user-sh</span><span class="invisible">ort-courses/esc7/</span></a> Topics: <a href="https://mastodon.social/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a> <a href="https://mastodon.social/tags/AFM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>AFM</span></a> <a href="https://mastodon.social/tags/superresolution" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>superresolution</span></a> <a href="https://mastodon.social/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a></p>
Francesca Bottanelli<p>A tour the force of <a href="https://mstdn.science/tags/geneediting" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>geneediting</span></a> and <a href="https://mstdn.science/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a>. We have followed up on some of these findings and have 2 papers that will drop soon! looking at endogenous proteins at high resolution in their own unperturbed cellular environment is helping to reveal important trafficking mechanisms! <br>---<br>RT <span class="h-card"><a href="https://mstdn.science/@JCellBiol" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@<span>JCellBiol</span></a></span><br>.@FreeThePipette, <span class="h-card"><a href="https://mstdn.science/@fran_bottanelli" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@<span>fran_bottanelli</span></a></span> and colleagues combine gene editing and STED super-resolution <a href="https://mstdn.science/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a> to reveal the nanoscale localizati…<br><a href="https://twitter.com/JCellBiol/status/1651620746806820872" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://</span><span class="ellipsis">twitter.com/JCellBiol/status/1</span><span class="invisible">651620746806820872</span></a></p>
Centriolelab<p>Image obtained with the @LeicaMicro SP8 STED <br>---<br>RT @louvel_vincent<br>First time doing <a href="https://mstdn.social/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> microscopy on Nuclear Pore Complexes with NUP96 staining, absolutely stunting 😵💫 <br><a href="https://twitter.com/louvel_vincent/status/1649061277895995394" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://</span><span class="ellipsis">twitter.com/louvel_vincent/sta</span><span class="invisible">tus/1649061277895995394</span></a></p>
Leica Microsystems<p>🔶 What are the additional insights you can gain from imaging tissue sample in 10 colors?<br>🔶 Follow our on-demand webinar with Dr. Nicolas Gaudenzio and discover how to perform 10-color imaging using a confocal microscope. See how this allows to assess the skin immune landscape.</p><p><a href="https://fcld.ly/lciceh8" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://</span><span class="">fcld.ly/lciceh8</span><span class="invisible"></span></a></p><p><a href="https://mastodon.social/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> <a href="https://mastodon.social/tags/STELLARIS" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STELLARIS</span></a> <a href="https://mastodon.social/tags/confocal" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>confocal</span></a> <a href="https://mastodon.social/tags/immune" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>immune</span></a></p>
Markus Osterhoff<p>Tim Salditt (<a href="http://twitter.com/SaldittLab" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">http://</span><span class="">twitter.com/SaldittLab</span><span class="invisible"></span></a>, substituting Jasper Frohn): <a href="https://academiccloud.social/tags/Multiscale" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Multiscale</span></a> <a href="https://academiccloud.social/tags/Xray" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Xray</span></a> Phase Contrast <a href="https://academiccloud.social/tags/Tomography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Tomography</span></a> at <a href="https://academiccloud.social/tags/GINIX" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>GINIX</span></a>/P10: Concepts, Implementation and Applications</p><p>- fantastic collaboration with the P10 team<br>- directly comparing <a href="https://academiccloud.social/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> to <a href="https://academiccloud.social/tags/Minflux" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Minflux</span></a>; old <a href="https://academiccloud.social/tags/confocal" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>confocal</span></a>? not worth mentioning 😂<br>- overview tomo, then zoom-in<br>- tumorous human pancreatic tissue <a href="https://academiccloud.social/tags/biopsy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>biopsy</span></a>: quantify the <a href="https://academiccloud.social/tags/tumor" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>tumor</span></a> type<br>- from electron density to metrics, quantify fibres etc.<br>- <a href="https://academiccloud.social/tags/hippocampus" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>hippocampus</span></a> patho punch, <a href="https://academiccloud.social/tags/Alzheimer" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Alzheimer</span></a> sample</p>
Spirochrome<p>Have a good <a href="https://mstdn.science/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>FluorescenceFriday</span></a> with this live HeLa cell imaged by <a href="https://mstdn.science/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a>. Actin in white and mitochondria in "Duo Intense Yellow" LUT by James Manton.</p><p> 🙏 Image Credit: Tianyan Liu</p><p><a href="https://mstdn.science/tags/PKmito" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>PKmito</span></a> <a href="https://mstdn.science/tags/SPYprobes" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>SPYprobes</span></a> <a href="https://mstdn.science/tags/imaging" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>imaging</span></a> <a href="https://mstdn.science/tags/lifesciences" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>lifesciences</span></a></p>
Spirochrome<p>📢New product launch!<br>We introduce SpyRho 555 from the lab of Murat Sunbul in Heidelberg. SpyRho 555 is a fluorogenic dye binding to RhoBAST aptamer for live or fixed cell RNA imaging. It works for widefield, confocal, SIM, STED and PAINT-like SMLM.</p><p>SpyRho 555 product page: <a href="https://spirochrome.com/product/spyrho/" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://</span><span class="ellipsis">spirochrome.com/product/spyrho</span><span class="invisible">/</span></a></p><p>SpyRho BioRxiv preprint link: <a href="https://www.biorxiv.org/content/10.1101/2022.10.24.513449v1" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/20</span><span class="invisible">22.10.24.513449v1</span></a></p><p>We thank Daniel Englert and <br>Murat Sunbul for providing the images and the helpful discussions.</p><p><a href="https://mstdn.science/tags/SPYprobes" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>SPYprobes</span></a> <a href="https://mstdn.science/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> <a href="https://mstdn.science/tags/imaging" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>imaging</span></a> <a href="https://mstdn.science/tags/RNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>RNA</span></a> <a href="https://mstdn.science/tags/LifeSciences" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>LifeSciences</span></a></p>
Spirochrome<p>📢The amazing PKmito RED, ORANGE and DEEP RED probes will be available next Monday (Jan. 16th) from all our distributors!</p><p>We will announce a cool promotion for the launch of the probes. Make sure to follow us to be in the loop next Monday!</p><p><a href="https://mstdn.science/tags/PKmito" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>PKmito</span></a> <a href="https://mstdn.science/tags/mitochondria" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>mitochondria</span></a> <a href="https://mstdn.science/tags/Imaging" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Imaging</span></a> <a href="https://mstdn.science/tags/lifesciences" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>lifesciences</span></a> <a href="https://mstdn.science/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> <a href="https://mstdn.science/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a></p>
Spirochrome<p>Just because we love PKmito ORANGE, here's a little bonus movie showing <a href="https://mstdn.science/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> <a href="https://mstdn.science/tags/LiveCellimaging" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>LiveCellimaging</span></a> of HeLa cells stained with PKmito ORANGE and mtDNA (picogreen). this movie is adapted from movie S3 in the following article from <span class="h-card"><a href="https://mstdn.science/@zhixingchen" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@<span>zhixingchen</span></a></span> lab: <a href="https://www.pnas.org/doi/10.1073/pnas.2215799119" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">pnas.org/doi/10.1073/pnas.2215</span><span class="invisible">799119</span></a></p><p><a href="https://mstdn.science/tags/mitochondria" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>mitochondria</span></a> <a href="https://mstdn.science/tags/lifesciences" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>lifesciences</span></a> <a href="https://mstdn.science/tags/PKmito" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>PKmito</span></a></p>
VicidominiLab<p>We designed a new method, focus-ISM, capable of achieving perfect optical sectioning in ISM. Furthermore, we combined <a href="https://mstdn.science/tags/ISM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>ISM</span></a> with <a href="https://mstdn.science/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> microscopy. The result is a <a href="https://mstdn.science/tags/microscope" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscope</span></a> with unbounded resolution and full background rejection.</p><p>(2/5)</p>
Ulrike Boehm<p>Are you unfamiliar with the terms <a href="https://mstdn.science/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a>, <a href="https://mstdn.science/tags/PALM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>PALM</span></a>, <a href="https://mstdn.science/tags/STORM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STORM</span></a>, <a href="https://mstdn.science/tags/SMLM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>SMLM</span></a>, <a href="https://mstdn.science/tags/MINFLUX" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>MINFLUX</span></a>, & <a href="https://mstdn.science/tags/MINSTED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>MINSTED</span></a>, but would you like to learn more about these <a href="https://mstdn.science/tags/superresolution" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>superresolution</span></a> <a href="https://mstdn.science/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a> technologies🔬? If so, then you should check out <a href="https://mstdn.science/tags/StefanHell" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>StefanHell</span></a>'s <a href="https://mstdn.science/tags/Lindau" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Lindau</span></a> presentation 🤓 Enjoy👉 <a href="https://mediatheque.lindau-nobel.org/recordings/39775" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://</span><span class="ellipsis">mediatheque.lindau-nobel.org/r</span><span class="invisible">ecordings/39775</span></a></p>
Francesca Bottanelli<p><a href="https://mstdn.science/tags/introduction" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>introduction</span></a> take 2! I post about <a href="https://mstdn.science/tags/imaging" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>imaging</span></a> <a href="https://mstdn.science/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> <a href="https://mstdn.science/tags/SuperResolution" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>SuperResolution</span></a> <a href="https://mstdn.science/tags/cellbio" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>cellbio</span></a> <a href="https://mstdn.science/tags/membranes" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>membranes</span></a> and anything ARF GTPases but also about the joy and struggles of bring a mother of 2 and <a href="https://mstdn.science/tags/newPi" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>newPi</span></a> in academia <a href="https://mstdn.science/tags/academicmum" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>academicmum</span></a>. I am currently on leave after the birth of n2 and trying to juggle running my lab and taking care of a baby with no sleep is the hardest thing I have ever done…</p>
Julien Delpierre<p>I came across this <a href="https://qoto.org/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a> paper. <a href="https://www.nature.com/articles/s41587-022-01519-4" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">nature.com/articles/s41587-022</span><span class="invisible">-01519-4</span></a><br>"By blue-shifting the <a href="https://qoto.org/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a> beam and separating <a href="https://qoto.org/tags/fluorophores" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>fluorophores</span></a> by on/off switching, individual fluorophores bound to a <a href="https://qoto.org/tags/DNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>DNA</span></a> strand are localized with σ = 4.7 Å, corresponding to a fraction of the fluorophore size, with only 2,000 detected <a href="https://qoto.org/tags/photons" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>photons</span></a> ."<br>This image showing resolution below the size of the fluorophore leaves me speechless.<br>The paper features images of <a href="https://qoto.org/tags/DNAorigami" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>DNAorigami</span></a>, <a href="https://qoto.org/tags/NuclearPores" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>NuclearPores</span></a>, synaptic <a href="https://qoto.org/tags/Vesicles" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Vesicles</span></a>, <a href="https://qoto.org/tags/ECM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>ECM</span></a> (<a href="https://qoto.org/tags/laminin" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>laminin</span></a>) and <a href="https://qoto.org/tags/vilmentin" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>vilmentin</span></a></p>
Jakob Rentsch<p>Hi <a href="https://det.social/tags/science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>science</span></a> mastodon! My work boils down to this question: Is membrane-proximal <a href="https://det.social/tags/actin" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>actin</span></a> generating <a href="https://det.social/tags/diffusion" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>diffusion</span></a> barriers for <a href="https://det.social/tags/membrane" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>membrane</span></a> molecules?</p><p>I am a PhD student of Helge Ewers at FU Berlin. I work in <a href="https://det.social/tags/neurons" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>neurons</span></a> because actin forms very well-behaved rings there. My approach is combining <a href="https://det.social/tags/SingleParticleTracking" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>SingleParticleTracking</span></a> of membrane proteins and <a href="https://det.social/tags/SuperResolution" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>SuperResolution</span></a> <a href="https://det.social/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a> of the <a href="https://det.social/tags/cytoskeleton" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>cytoskeleton</span></a> via <a href="https://det.social/tags/STORM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STORM</span></a> and <a href="https://det.social/tags/STED" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>STED</span></a>. Lots of <a href="https://det.social/tags/biophysics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>biophysics</span></a> , <a href="https://det.social/tags/CellBiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>CellBiology</span></a> and <a href="https://det.social/tags/neuroscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>neuroscience</span></a>. <a href="https://det.social/tags/introduction" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>introduction</span></a> <a href="https://det.social/tags/ScienceMastodon" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>ScienceMastodon</span></a></p>
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