Cosmin Saveanu<p>Seeing <a href="https://framapiaf.org/tags/mRNAs" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>mRNAs</span></a> in cells, often involves the addition of MS2 binding sites in the 3' <a href="https://framapiaf.org/tags/UTR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>UTR</span></a> that can be detected with fluorescent <a href="https://framapiaf.org/tags/MS2" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>MS2</span></a> coat protein. The MS2 binding site repeats increase the size of the 3' UTR and can also affect the degradation of <a href="https://framapiaf.org/tags/RNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>RNA</span></a> fragments containing them: <a href="https://pubmed.ncbi.nlm.nih.gov/30218101/" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://</span><span class="ellipsis">pubmed.ncbi.nlm.nih.gov/302181</span><span class="invisible">01/</span></a></p><p>Longer 3' UTR induce the degradation of the RNA of interest through nonsense-mediated mRNA decay (NMD). This can be avoided by tethering <a href="https://framapiaf.org/tags/eRF3" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>eRF3</span></a> or a mutant <a href="https://framapiaf.org/tags/PAB1" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>PAB1</span></a>.<br><a href="https://www.biorxiv.org/content/10.1101/2022.02.05.479257v1.full" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/20</span><span class="invisible">22.02.05.479257v1.full</span></a></p>